SMDC994566 (KRAS4b inhibitor Compound 2) is a specific small molecule covalent modifier of canonical RAS isoform KRAS4b CaaX-box cysteine (C185), decreases proliferation and KRAS protein level in KRAS-dependent MEF cells.
SMDC994566 (KRAS4b inhibitor Compound 2) is a specific small molecule covalent modifier of canonical RAS isoform KRAS4b CaaX-box cysteine (C185), decreases proliferation and KRAS protein level in KRAS-dependent MEF cells. Compound 2 does not modifies C186 in NRAS. Compound 2 (0-50 uM) inhibits the proliferation of KRAS4b mutant MEFs but does not inhibit the growth of Myr-KRAS G12D/C185S MEFs. Compound 2 (0-50 uM) also reduces KRAS protein levels in KRAS4b G12D and G12C but not in Myr-KRAS G12D/C185S MEFs after 72 h of treatment.
